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For example, a specific siRNA can be used for post-transcriptional silencing of specific mRNA targets Dorsett and Tuschl 2004. Target specificity in RNAi is achieved through RNA-RNA sequence recognition and base pairing. The siRNA consists of double stranded RNA, typically of 19-21 base pair long, with two nucleotides overhanging at each 3' end. For maximal stability, two 2' deoxynucleotides are used as 3' overhangs. Alternatively, 27-mer blunt-ended nucleotides may be used, as these have shown improved efficiency in gene silencing Kim, Behlke et al. 2005. Transport of siRNA into cells may be enhanced by encapsulation into liposomes or by covalent coupling to highly lipophilic agents. Soutschek et al 2004.
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